The CBC parameters are obtained using DC detection (RBC/PLT), SLS method (HGB) and flow cytometry (WBC). When doing a CBC, the XN-L uses a technology that allows reliable determination and accurate counts of WBC since it effectively avoids interferences from lyse-resistant RBC and non-cellular particles, such as debris or lipids. In addition to the red blood cell count, the XN-L CBC provides information on the ratio of microcytes and macrocytes.
All differential WBC parameters including IG are obtained using fluorescence flow cytometry. The DIFF measurement provides an excellent flagging sensitivity and counting by special shape recognition analysis of each subpopulation and flexible gating for counting. It delivers a greater range of clinically relevant parameters, such as the immature granulocytes (IG) count in every differential analysis that is performed. The sensitivity of the XN-DIFF is particularly useful for detecting inflammations or infections. It also helps you monitor therapies – elevated white blood cell counts going down can show therapies are working.
The optional ‘RET’ APP delivers automated reticulocyte counting. The reticulocyte count using our proprietary fluorescence flow cytometry methodology is recognised for its accuracy around the world. In fact, it has been used in doping control for high-profile events like the Tour de France since 2006.
Using the RET APP, you can analyse the combined information of reticulocyte parameters – both related to quality and quantity – and so deliver the whole picture. You also have significantly faster control during iron and/or erythropoietin therapy. And most importantly, patients have a greater chance of recovery. Alongside the pure reticulocyte count, the XN- L Series RET application provides reticulocyte fractions (IRF, LFR, MFR, HFR) that reflect maturity stages and therefore erythropoietic activity, and additional information on the quality of newly formed red blood cells by reporting the degree of reticulocyte haemoglobinisation (RET-He), an advanced clinical parameter that helps to manage iron-deficient and anaemic patients.
Additionally, RET provides information on hypo- and hyperchromic red blood cells and fragmented red cells. It automatically calculates the reticulocyte production index (RPI). For consistent result quality, the PLT-O parameter provides accurate platelet counts even in the event of common interferences. Without the need for manual counting.
Unique to this class of analysers, the XN-L Series analysers can handle more than your blood samples. The optional ‘XN-BF’ APP lets you measure body fluids automatically and straight from the tube. You don‘t need to specially prepare the sample and the results provide valuable information on the possible cause of inflammations – fast.
You can select an automated body fluid mode as an option, dedicated to analysing cerebrospinal fluid (CSF), synovial, and serous (e.g. pleural, CAPD or ascites) fluids. Anyone who has analysed body fluids knows the sensitivity of the process. We have tried to address all the critical aspects.
Without sample pre-treatment, a special automatically activated counting sequence provides a precise total count of nucleated cells (TNC). It works even in the lowest counting ranges, such as applicable for CSF. To enhance accuracy further and help deliver better treatments, the XN-L Series has a three-fold counting volume compared to a standard counting chamber. Measuring CSF can quickly indicate bacterial meningitis from the white blood cell count (WBC-BF) plus differentiation into polymorphonuclear and mononuclear cells.